POLYPEPTIDE UNSTABILIZING PROTEIN IN CELLS UNDER AEROBIC CONDITIONS AND DNA ENCODING THE SAME

摘要

DNA encoding a polypeptide (LDLEMLAPYIPMDDDFQL) (I), or encoding a fusion protein containing at least 16 residues of (I), a nuclear localization signal, and another protein, and imparting relative stability under specific conditions of oxygen concentration within the cell, is new. Independent claims are also included for the following: (1) Vectors containing the DNA; (2) Cells transformed by the vectors; (3) Producing the fusion protein by culture of the transformed cells; (4) Detecting hypoxic conditions in cells by monitoring the stability of the protein fused to (I) in cells transformed by vectors containing the DNA; (5) Regulating the stability of proteins within the cell by transformation with the DNA; (6) Inhibiting the development of cells under hypoxic conditions, using the fusion protein; (7) Fusion proteins encoded by the DNA; and (8) Stabilization of cells under specific conditions of oxygen tension. ACTIVITY : Cytostatic. No biological data given. MECHANISM OF ACTION : Stabilization of a cytotoxic protein within cells in hypoxic regions of a solid tumor; Gene Therapy. No biological data given.