A process for generating genetically modified pearl millet through agrobacterium and biolistic transformation.

摘要

The present invention relates to a process for biolistic transformation and regeneration of Pennisetum glaucum (Pearl millet) comprising: a) initiating embryogenic calli formation from the seeds of P. glaucam in an MS media containing 5mg/L of 2,4 D; b) incubating the said calli in dark for a predetermined period; c) sub-culturing the calli on an MS media containing 3mg/L of 2,4 D; d) incubating said sub-cultured calli under light for a predetermined period; e) subjecting the embryogenic calli to biolistic bombardment with plasmid DNA containing pre-identified genes using a biolistic apparatus; f) allowing the proliferating calli to grow and differentiate into plantlets; g) analysing the expression of said pre-identified genes in the regenerated plantlets using known techniques.