АНТИТЕЛА К ФАКТОРУ HLDF, СПОСОБЫ ИХ ПОЛУЧЕНИЯ, ПЕПТИД С АНТИГЕННЫМИ И HK-ГИДРОЛИЗУЮЩИМИ СВОЙСТВАМИ И СПОСОБ ДИАГНОСТИКИ АНАПЛАСТИЧЕСКОГО СОСТОЯНИЯ КЛЕТКИ ЧЕЛОВЕКА

摘要

FIELD: biotechnology, peptides. ^ SUBSTANCE: invention relates to a method for preparing antibodies raised to human leukocyte differentiation factor (HLDF) or to HLDF fragment (31-38) representing peptide of the following structure: Arg-Arg-Trp-His-Arg-Leu-Glu-Lys possessing with antigenic and nucleic acids-hydrolyzing properties, and for diagnostic aims also. Antibodies are prepared from rabbit plasma blood immunized with three injections of antigens wherein synthetic HLDF factor or conjugate is used as antigens. Diagnosis of anaplastic state of human cells is carried out by using solutions of antibodies to HLDF factor or HLDF fragment (31-38) in the concentration 0.0013 mg/ml as biological markers. Invention provides carrying out the differential diagnosis of tumors and normal organs and effective detecting initial stages in cell differentiation disturbances. ^ EFFECT: improved preparing method of antibody, improved method for diagnosis. ^ 6 cl, 21 dwg, 1 tbl