PROTEIN PURIFICATION METHOD

摘要

Problem: To provide a method of purifying a physiologically active protein ( in particular, an antibody) whereby impurities such as DNA contaminants and viruses can be surely removed by a simple procedure at a low cost with littl e loss of the physiologically active protein. Means for Resolution: A method o f removing impurities in a sample containing a physiologically active protein which comprises the following steps: 1) preparing an aqueous solution of the physiologically active protein-containing sample having a conductivity at a pH value lower than the isoelectric point of the physiologically active protein ; and 2) removing the thus formed particles.