MULTIPLEX STANDARDIZED REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION METHOD FOR ASSESSMENT OF GENE EXPRESSION IN SMALL BIOLOGICAL SAMPLES

摘要

A method for direct comparison of numeral gene expression values between samples of genes using reverse transcription-polymerase chain reaction is described. CDNA, a competitive template mixture, and primer pairs for a plurality of genes are combined with at least one suitable buffer and at least one suitable enzyme to form a mixture. The mixture is amplified for a predetermined number of cycles to form PCR products. The PCR products are mixed with at least one suitable buffer, at least one enzyme, and one primer pair specific for each of the genes. The resulting mixture is amplified an additional predetermined number of cycles.