| 摘要 |
The present invention is related to a method for in vitro culture of mammalian ovarian follicles for bioassay purposes, comprising the following subsequent steps:-providing a suitable container for the in vitro culture,-selecting a follicle from an ovary of a mammal, said follicle comprising at least a theca or pre-theca cell, a granulosa cell and an oocyte,-an optional first culture step using an attachment prohibiting first medium free from oil,-a second culture step using an attachment promoting second medium free from oil arranged to, and-the retrieval of the matured follicle.
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