发明名称 |
NOVEL CELL-BASED ASSAYS FOR G-PROTEIN-COUPLED RECEPTOR-MEDIATED ACTIVITIES |
摘要 |
Disclosed are compositions and methods for their use, such as identifying G-protein-coupled receptors, ligands and compounds that modulate the activities of G-protein-coupled receptors. The compositions and methods employ cyclic nucleotide-gated channels and fluorescence dyes in detecting changes of intracellular cAMP levels in response to the stimulation of G-protein-coupled receptors. Activation of the G-protein-coupled receptors can be detected in a variety of assays, including cell-based imaging assays with fluorescence microscopes and high throughput assays with multi-well plates and fluorescence plate readers. |
申请公布号 |
WO03038039(A3) |
申请公布日期 |
2004.03.18 |
申请号 |
WO2002US34122 |
申请日期 |
2002.10.25 |
申请人 |
APTUS PHARMACEUTICALS, INC. |
发明人 |
YAO, YONG;CAO, LIANG |
分类号 |
G01N33/50;C12N5/10;C12N15/09;C12Q1/02;G01N21/78;G01N33/15;G01N33/566 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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