发明名称 |
METHOD FOR ISOLATING AND CULTURING UNCULTURABLE MICROORGANISMS |
摘要 |
The invention provides a method for isolating and culturing a previously unculturable microorganism, which comprises: (i) collecting a sample from an environmental source; (ii) counting/estimating the number of microorganisms in the sample; (iii) diluting the sample in an appropriate medium; (iv) adding a gelating agent such as to entrap one or more microorganisms within a sphere of the gelating agent; (v) coating the spheres containing the entrapped microorganism(s) with a natural or synthetic polymer to form a polymeric membrane; (vi) incubating the coated spheres in the original environment for an appropriate time; (vii) cutting the spheres and scanning for microorganisms colonies; and (viii) isolating the microorganisms, and repeating steps (iii) to (vii) until a pure clone of said previously unculturable microorganism is obtained. |
申请公布号 |
WO2004022698(A2) |
申请公布日期 |
2004.03.18 |
申请号 |
WO2003IL00725 |
申请日期 |
2003.09.03 |
申请人 |
BEN GURION UNIVERSITY OF THE NEGEV RESEARCH AND DEVELOPMENT AUTHORITY;KUSHMARO, ARIEL;GERESH, SHIMONA |
发明人 |
KUSHMARO, ARIEL;GERESH, SHIMONA |
分类号 |
C12N1/00;C12P21/04 |
主分类号 |
C12N1/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|