DEVICE FOR CONTROLLED DRYING AND DERIVATIZATION IN PLANAR CHROMATOGRAPHY AND ITS APPLICATION

摘要

The inventive device allows to substantially improve the reproducibility of measurements in quantitative thin-layer chromatography (TLC and HPTLC). Accuracy, sensitivity and reproducibility are improved by eliminating the uncontrolled band broadening resulting from diffusion, nonuniform vertical in-depth distribution of spots in the sorbent layer during drying, and nonhomogeneous derivatization conditions. The main characteristics of the inventive device are as follows: the heated air flow is directed over the sorbent layer in the direction of chromatogram development. The temperature of the heated air is preferably not constant but regularly oscillating between a minimum and a maximum value. The range between the minimum and the maximum temperature values, as well as the length of the oscillation interval, may be regulated using a dedicated program. The device also supports computerized drying and automated operation through integration into a complete system for processing and evaluating chromatograms and results.