New polynucleotide encoding mutant human glucose transporter, useful for identifying antidiabetic agents that can be used to treat diabetes types I or II

摘要

A purified, isolated polynucleotide (I) that contains a DNA sequence encoding the human glucose transporter protein, GLUT4Val85Met (II), is new. Independent claims are also included for the following: (1) Saccharomyces cerevisiae cell in which all glucose transporters are no longer functional and no functional Erg4 protein is present; (2) method for preparing yeast cells that contain (I); (3) yeast cells in which all glucose transporters are no longer functional and which contain (I); (4) method for identifying compounds (A) that stimulate activity of GLUT4 protein; and (5) methods for identifying compounds (B) or (C) that inhibit the proteins encoded by the Fgy1 and ERG4 genes, respectively.