| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for producing an immobilized enzyme for esterification reaction with high activity, by controlling a water content of the enzyme, without conducting drying of the enzyme which causes deactivation of the enzyme. SOLUTION: This method for producing the immobilized enzyme for the esterification reaction comprises making a fat and oil degradative enzyme adsorbed and immobilized on an immobilizing carrier, then bringing the enzyme on the carrier into contact with a triglyceride of a fatty acid or a partial triglyceride of the fatty acid, without drying the enzyme, so that a residual water content of the enzyme is controlled to be 5-50 wt% based on the weight of the carrier. Further, the immobilized enzyme for the esterification reaction is produced by making the fat and oil degradative enzyme adsorbed and immobilized on the immobilizing carrier, then dehydrating the enzyme, without directly drying the enzyme, while bringing the enzyme on the carrier into contact with a fatty acid, the triglyceride of the fatty acid or the partial triglyceride of the fatty acid in an amount of 20-3000 wt% based on the weight of the carrier, so that the residual water content of the enzyme is controlled to be 5-50 wt% based on the weight of the carrier. COPYRIGHT: (C)2004,JPO
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