Regeneration method of immobilized enzyme

摘要

Regenerating (M1) an immobilized enzyme for lipolysis which exhibits a reduced activity after been used for lipolysis, involves washing the immobilized enzyme having fatty acids with a solvent, controlling an equilibrium concentration of the fatty acids in the solvent, removing the washed immobilized enzyme and contacting the resulting immobilized enzyme with a fresh enzyme, adsorbing the fresh enzyme onto the immobilized enzyme.