METHOD FOR REFOLDING IGF-1 TO ACTIVE CONFORMATION

摘要

A method for refolding insoluble, improperly folded IGF-I is provided, where in the IGF-I, precipitated from prokaryotic host cells, is concurrently solubilized, unfolded, and refolded into a biologically active conformation in a single buffer. The buffer contains reducing agent and chaotropic agent to solubilize the IGF-1 at concentrations sufficiently low to allow solubilization and refolding to occur. Also provided is a triple-protease deficient E. coli hos t suitable for use in the process.