发明名称 |
METHODS FOR PRODUCING MAMMALIAN TRYPSINS |
摘要 |
<p>The present invention relates to methods for producing a mammalian trypsin in a Fusarium venenatum host strain, comprising (a) cultivating the Fusarium venenatum host strain in a culture medium under conditions suitable for expression of the mammalian trypsin and secretion thereof into the medium, wherein the Fusarium venenatum host strain comprises a nucleic acid construct comprising a nucleic acid sequence encoding the mature coding sequence of a mammalian trypsin operably linked to nucleotides 58 to 129 of SEQ ID NO: 1 encoding the signal peptide and propeptide of Fusarium oxysporum trypsinogen; and (b) recovering the mammalian trypsin from the medium. The present invention also relates to constructs comprising a nucleic acid sequence encoding the mature coding sequence of a mammalian trypsin operably linked to nucleotides 58 to 129 of SEQ ID NO: 1 encoding the signal peptide and propeptide of Fusarium oxysporum trypsinogen, and to vectors and Fusarium venenatum host strains comprising such constructs.</p> |
申请公布号 |
WO2004020612(A1) |
申请公布日期 |
2004.03.11 |
申请号 |
WO2003US27074 |
申请日期 |
2003.08.29 |
申请人 |
NOVOZYMES BIOTECH, INC. |
发明人 |
BERKA, RANDY;BROWN, KIMBERLY |
分类号 |
C12N1/15;C12N9/64;C12N9/76;C12N15/80;(IPC1-7):C12N1/15;C12N15/63;C12P21/00;C12N15/00 |
主分类号 |
C12N1/15 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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