Detecting analyte using luminescent metal complex, useful for diagnosis, where chemiluminescence is induced by sequential oxidation and reduction of the complex

摘要

Detecting an analyte (I) using a luminescent metal complex (A) as marker in which chemiluminescence of (A) is excited by oxidation of (A), reduction of the complex with nascent hydrogen to induce chemiluminescence and determination of (I) from this chemiluminescence, is new. Independent claims are also included for the following: (1) device for the method that comprises an oxidizing agent for (A), system for generating nascent hydrogen and system for detecting chemiluminescence; (2) method for generating chemiluminescence by sequential oxidation and reduction, with nascent hydrogen, of a suitable metal complex; and (3) device for generating chemiluminescence comprising oxidizing agent for (A) and system for generating nascent hydrogen.