发明名称 |
CHROMATOGRAPHIC TWO-LAYER PARTICLES |
摘要 |
The present invention is a method of producing a chromatographic separation matrix, wherein porous polymeric particles comprised of two layers with different properties are prepared in a two-phase system by (a) providing porous polymeric particles with reactive groups on their surfaces; (b) washi ng said particles with a first solvent and draining the particles to enclose a first phase; (c) wetting the outer layer of the particles by adding a second solvent, which is essentially insoluble in the first solvent; (d) reacting t he reactive groups in the outer layer by adding a reagent, which is essentially non-reactive in the first solvent; and (e) coupling of chromatographic bindi ng groups to the reactive groups in the inner layer. The invention also encompasses a porous polymer particle suitable for use as a chromatographic separation matrix and a process of separation, wherein a matrix according to the invention is used.
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申请公布号 |
CA2495491(A1) |
申请公布日期 |
2004.03.11 |
申请号 |
CA20032495491 |
申请日期 |
2003.08.18 |
申请人 |
AMERSHAM BIOSCIENCES AB |
发明人 |
CARLSSON, MATS;BUSSON, PHILIPPE;BERG, HANS |
分类号 |
B01J20/281;B01D15/18;B01J20/28;B01J20/285;B01J20/286;G01N30/00;G01N30/88;(IPC1-7):G01N30/48;C08J7/02;B01D15/08;C08J7/12;B01J20/22;B01J20/30;G01N30/50 |
主分类号 |
B01J20/281 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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