发明名称 |
AN ENZYMATIC PROCESS FOR PREPARING BETA-LACTAMS |
摘要 |
An enzymatic process under kinetical control for preparing b-lactams, by coupling the 7-amino group of a cephalosporin nucleus with the carboxylic function of an acetic acid derivative consisting of a residue with p-electrons, a short spacer and a carboxylic function activated as methyl- or ethylester. The process comprises using a penicillin amidase or a-amino acid esterase as free enzyme or in any suitable immobilised form; applying at least 100 mmol/l cephalosporin nucleus as long as it is soluble, otherwise 50 - 100 % of its maximum solubility is charged, applying the acetic acid activated as ester in a 1 - 3 fold molar ratio, and adjusting the pH to 6.0 - 8.0 and the temperature to 0 - 30 DEG C. |
申请公布号 |
WO2004020650(A2) |
申请公布日期 |
2004.03.11 |
申请号 |
WO2003EP09659 |
申请日期 |
2003.08.29 |
申请人 |
BIOFERMA MURCIA, S.A.;GIESECKE, ULRICH, EBERHARD |
发明人 |
GIESECKE, ULRICH, EBERHARD |
分类号 |
C12P35/00;C12P35/02;C12P35/04 |
主分类号 |
C12P35/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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