摘要 |
The invention relates to the field of ecology, biotechnology, molecular biology, biological chemistry, immunology, in particular to detection of components with the help of specific interactions between the latter and a microcarrier comprising a receptor immobilized thereon for defining said components. Increasing the reliability of simultaneous detection of a number of components in a mixture, making the analysis cheaper and less time-consuming, extending the range of markers used as well as providing the possibility of visual registration of the contents of a number of components in a mixture is achieved by a method and a device for simultaneous detection of a number of components in a mixture, said method comprising a step of reacting of the components under analysis with the specific receptors immobilized on the surfaces of microcarrier of various forms. As a result of formation of specific complexes a mobility of microcarrier under the influence of a physical field is changed. Due to the change of mobility of microcarrier under the influence of a physical field a separation into a fraction of non-reacted microcarrier with unchanged mobility, and a fraction or fractions of microcarrier with changed mobility. Since each type of a specific receptor is bound to its marked microcarrier the analysis of just a marker characteristic of microcarrier with changed mobility under the influence of a physical field will reveal the availability of the component which has reacted with this microcarrier in the mixture under analysis. Under a physical field as in the case described a gravitation field, an electric field or a magnetic field as well as combinations of the above is understood. A more efficient reacting of the mixture components with a minimum amount of microcairiers, provision of an efficient and operation-saving separation of microcarrier is achieved in a convenient for sterilization separating chamber where the process of retaining the microcarrier on the walls of a separating chamber and removing them into the collector is actually continuous.
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