发明名称 |
MEMBRANE-BASED ASSAYS USING TIME-RESOLVED FLUORESCENCE |
摘要 |
A membrane-based assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes time- resolved fluorescence to detect the signals generated by excited fluorescent labels. Because the labels can have relatively long emission lifetime, short - lived background interference can be practically eliminated through delayed fluorescence detection. In addition, the resulting fluorescent reader can ha ve a simple and inexpensive design. For instance, in one embodiment, the reader can utilize a silicon photodiodide and a pulsed light-emitting diode (LED) t o accurately excite labels and detect fluorescence on a membrane-based assay device without requiring the use of expensive components, such as monochromators or narrow emission band width optical filters.
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申请公布号 |
CA2495206(A1) |
申请公布日期 |
2004.03.11 |
申请号 |
CA20032495206 |
申请日期 |
2003.07.10 |
申请人 |
KIMBERLY-CLARK WORLDWIDE, INC. |
发明人 |
WEI, NING;SONG, XUEDONG;KNOTTS, MICHAEL;KAYLOR, ROSANN |
分类号 |
G01N21/27;G01N21/64;G01N33/52;G01N33/58;(IPC1-7):G01N33/543 |
主分类号 |
G01N21/27 |
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