PRODUCTION OF MULTIMERIC FUSION PROTEINS USING A C4BP SCAFFOLD

摘要

The present invention provides a method for obtaining a recombinant fusion protein comprising a scaffold of a C-terminal core protein of C4bp alpha chain, said recombinant fusion protein being capable of forming multimers in soluble form in a prokaryotic host cell, the method including the steps of ( i) providing a prokaryotic host cell carrying a nucleicacid encoding said recombinant protein operably linked to a promoter functional in said prokaryotic cell; (ii) culturing the host cell under conditions whereinsaid recombinant protein is expressed; and (iii) recovering the recombinant prote in wherein said protein is recovered in multimeric form without performing a scaffold refolding step.