Homogeneous fluorescent assay for kinase, phosphatase or phosphodiesterase, useful in screening for pharmaceuticals and plant-protection agents, uses polycationic polymer as quencher

摘要

Homogeneous assay for quantitative measurement of kinase, phosphatase or phosphodiesterase reactions by reacting the enzyme with a fluorescent, (de)phosphorylatable substrate (A) in presence of polycationic polymer (B) containing quencher groups. The change in phosphorylation is determined from a change in fluorescence.