PRIMER AND PROBE FOR DETECTING VIBRIO VULNIFICUS AND DETECTION METHOD USING THE SAME

摘要

It is intended to construct a specific gene amplification primer to be used for detecting, quantifying and identifying Vibrio vulnificus which is practically satisfactory in amplification efficiency and amplification specificity with little risk of miss judgment. Partial base sequences of gyrB gene, rpoD gene and recA gene of Vibrio vulnificus and its analog are determined and the systemic relation among them is clarified. Thus, bases characteristic to Vibrio vulnificus are identified so as to enable the design of a highly specific probe containing the same and a gene amplification primer having a high specificity and an excellent amplification efficiency.