发明名称 |
Label-free detection of single nucleotide polymorphisms using surface plasmon resonance imaging |
摘要 |
Disclosed are methods of using structure-specific, invasive nucleic acid cleavage reactions, in an addressed array format, to detect polymorphisms in general, and single nucleotide polymorphisms in particular. In the preferred embodiment of the invention, the addressed array format utilizes surface plasmon resonance imaging as a means for detecting the products of the invasive cleavage reaction and hence the presence of polymorphisms in the target nucleic acid. Sensitivity of the SPR detection is further enhanced by rolling circle amplification of the bound cleavage products.
|
申请公布号 |
US2004048287(A1) |
申请公布日期 |
2004.03.11 |
申请号 |
US20030441732 |
申请日期 |
2003.05.20 |
申请人 |
SMITH LLOYD M.;SHORTREED MICHAEL R.;CORN ROBERT M.;CHEN YAN |
发明人 |
SMITH LLOYD M.;SHORTREED MICHAEL R.;CORN ROBERT M.;CHEN YAN |
分类号 |
C12P19/34;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12P19/34 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|