发明名称 |
CIRCULAR NUCLEIC ACID VECTORS, AND METHODS FOR MAKING AND USING THE SAME |
摘要 |
Circular nucleic acid vectors that provide for persistently high levels of protein expression are provided. The circular vectors of the subject invention are characterized by being devoid of expression-silencing bacterial sequences, where in many embodiments the subject vectors include a unidirectional site-specific recombination product hybrid sequence in addition to an expression cassette. Also provided are methods of using the subject vectors for introduction of a nucleic acid, e.g., an expression cassette, into a target cell, as well as preparations for use in practicing such methods. The subject methods and compositions find use in a variety of different applications, including both research and therapeutic applications. Also provided is a highly efficient and readily scalable method for producing the vectors employed in the subject methods, as well as reagents and kits/systems for practicing the same. |
申请公布号 |
WO2004020605(A2) |
申请公布日期 |
2004.03.11 |
申请号 |
WO2003US27294 |
申请日期 |
2003.08.28 |
申请人 |
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY;KAY, MARK, A.;CHEN, ZHI-YING |
发明人 |
KAY, MARK, A.;CHEN, ZHI-YING |
分类号 |
C12N15/63;A61K39/00;A61K48/00;C12N5/10;C12N15/00;C12N15/85 |
主分类号 |
C12N15/63 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|