| 摘要 |
The invention relates to methods for diagnosing Alzheimer's disease from a blood specimen. The method involves incubating a blood sample with a fluorescent probe that interacts with either protein kinase C (PKC) or protein kinase A (PKA), and measuring the fluorescence intensity in different conditions. Variation in the emission spectra data relative to those of the spectra obtained with healthy subjects is a discriminatory factor vis-à-vis Alzheimer's disease. The probes may recognize the catalytic site or the regulator domain of PKC or PKA, and may be coupled to a synthetic organic compound such as bis-indolmaleimide. In one aspect, the probes are lipidic, including probes prepared from phospholipid constituents of a cell membrane. In another aspect, the probes are proteinic or peptide probes of a membrane or cytoskeletal nature. Useful probes include derivatives of substrates for PKC or PKA.
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