GLYCOSYLATED HUMAN INTERFERON ALPHA ISOFORM

摘要

PURPOSE: A glycosylated human interferon alpha isoform is provided, which has improved stability in human body and reduces dose and dosage frequency thereof when clinically applied. CONSTITUTION: A glycosylated human interferon alpha isoform is prepared by forming one or more sequences of Asn-X-Ser/Thr(N-X-S/T) on the amino acid residues; -Cys1-Ser8 (Cys1-Asp-Leu-Pro-Gln-Thr-His-Ser8); -Arg22-Thr52 (Arg22-Arg-Ile-Ser-Leu-Phe-Ser-Cys-Leu-Lys-Asp-Arg-His-Asp-Phe-Gly-Phe-Pro-A sn-Glu-Glu-Phe-Gly-Asn-Gln-Phe-Gln-Lys-Ala-Glu-Thr52); -Ser68; -Asp77; -Lys134-Ser137 (Lys134-Tyr-Ser137), and -Gln158-Glu165 (Gln158-Glu-Ser-Leu- Arg-Ser-Lys-Glu165), and followed by glycosylation, wherein leucine at amino acid position 26 is substituted by asparagine, histidine and phenylalanine at amino acid position 34 and 36 are substituted by asparagine and serine, respectively, lysine at amino acid position 134 is substituted by asparagine, or all of them are modified.