MOLLUSK DERIVED GROWTH FACTOR(MDGF) FOR STIMULATING NEURITE OUTGROWTH AND PROTEIN FRACTION ISOLATED FROM APLYSIA HEMOLYMPH

摘要

PURPOSE: A mollusk derived growth factor(MDGF) for stimulating neurite outgrowth and a protein fraction isolated from Aplysia hemolymph are provided, thereby reviving neurite and increasing memory of a person. CONSTITUTION: A mollusk derived growth factor(MDGF) for stimulating neurite outgrowth is derived from Aplysia californica. A purification method of protein fraction for stimulating neurite outgrowth from hemolymph comprises the steps of: (a) extracting Aplysia hemolymph; slowly adding 40 to 60% of ammonium sulfate into the hemolymph to dissolve the hemolymph; (c) centrifuging the hemolymph-dissolved solution to obtain a precipitation; and (d) dialysis of the precipitation to remove ammonium sulfate.