| 摘要 |
A newly invented method for plant gene transfer by ovary injection and micro-vibration is described. First the stigma is cut off at a suitable interval of time after pollination. A tiny DNA passage is then defined by penetrating the injection needle from the position of the cut-off at the style and toward the locule inside the ovary, or further penetrating through the ovary wall near the receptacle. The exotic DNA (deoxyribonucleic acid) containing gene(s) to be transferred is injected into the locule via the passage. After injection, the ovule inside the ovary is surrounded by the exotic DNA in the locule. The ovary is then merged in water which is vibrated by a micro-vibration treatment. The micro-vibration can be created by ultra-sonic device. Under good timing management, the micro-vibration treatment will enable the injected DNA to have higher ability to enter the ovule and encounter with the fertilizing egg. This will increase the possibility of DNA recombination, i.e. gene transfer, between exotic DNA and the fertilized egg's chromosome. The transgenic fertilized egg will then naturally develop into a transgenic seed. Finally a whole transgenic plant can be obtained from a transgenic seed through germination and vegetative growth. The whole procedures are simple and easy to do, and does not need the aid of tissue culture. The method in this invention has high value and potential for practical application, since it is very simple, inexpensive, with high efficiency in plant's genetic improvement, and benefiting the environmental safety.
|
