PRIMER FOR DETECTING MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS AND METHOD FOR DIAGNOSING JOHNE'S DISEASE BY USING THE PRIMER

摘要

PROBLEM TO BE SOLVED: To provide a primer capable of efficiently amplifying a specific base sequence on an insertion sequence IS900 (sequence No.1) of Mycobacterium avium subs. Paratuberculosis, and to provide a simple method for genetically diagnosing Johne's disease by using the primer.

SOLUTION: This new primer amplifies the base sequence of a target region selected from the insertion sequence IS900 (sequence No.1) of the Mycobacterium avium subs. Paratuberculosis or its complementary chain, wherein the primer contains (1) a base sequence which functions as a primer by annealing the specific base sequence on the insertion sequence IS900 of the Mycobacterium avium subs. Paratuberculosis as a first region and (2) another base sequence which comprises a sequence complementary to a base sequence of the 3' side of the first region and positions on the 5' side of the first region as a second region. Further, a method for amplifying the specific base sequence on the insertion sequence IS900 of the Mycobacterium avium subs. Paratuberculosis is conducted by utilizing a LAMP method in which the primer is used.

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