Method for plant gene transfering by electrical shock and ovary injection

摘要

A method for plant gene transfer by ovary injection and electrical shock. First the stigma is cut off after the plant is pollinated. A tiny DNA passage is then defined by penetrating an injection needle from the cut-off at the style and toward the locule inside the ovary. An exotic DNA containing gene(s) is injected into the locule via the passage. An electric field is set up with the center of the ovary being negative and the periphery positive. The DNA which is negatively charged in nature will move outward in such an electric field. This arrangement increases possibility of DNA recombination.