ANTI-ROTAVIRUS ANTIBODY, IMMUNOGLOBULIN YOLK/EGG(IGY/EGG) CONTAINING THE ANTI-ROTAVIRUS ANTIBODY, AND METHOD FOR PREPARING THE SAME IGY/EGG

摘要

PURPOSE: An anti-rotavirus antibody, immunoglobulin yolk/egg(IgY/egg) containing the anti-rotavirus antibody, and a method for preparing the IgY/egg are provided, thereby producing IgY/egg having high titer and effectively inhibiting rotavirus. CONSTITUTION: An anti-rotavirus antibody composition shows specificity to a rotavirus antigen VP8, wherein the anti-rotavirus antibody composition further shows specificity to a rotavirus antigen VP5 and one or more antigens selected from rotavirus antigen VP4, rotavirus antigen VP7 and rotavirus; and the rotavirus is Rotavirus KRV01(KGCC-11313). A method for preparing IgY/egg containing the anti-rotavirus antibody comprises the steps of: culturing rotavirus; preparing cDNA corresponding to an antigen VP8 from rotavirus RNA; preparing dsDNA from the cDNA; digesting dsDNA and inserting the digested dsDNA into a plasmid; inserting the plasmid into E. coli; expressing the antigen gene in the plasmid to produce an antigen; administrating the antigen into a laying chicken; and obtaining IgY/egg from the immunized chicken.