Identification of human tissue using optical spectroscopy

摘要

Tissue types (e.g. tumorous or normal) are determined using optical spectroscopy. Autofluorescence and diffuse reflectance spectra are generated by separately illuminating a tissue surface area with monochromatic light and white light. A peak in autofluorescence intensity (F) is provided around 460 nm from both from normal and tumorous human brain tissue with 337 nm monochromatic light excitation. Separation between white/gray matter and brain tumors is provided by certain combined F-Rd spectrum numerical values, especially certain ratios of F and Rd between 400 nm-600 nm. Numerical values based on certain combinations of unequal exponential powers of F and Rd are essentially unaffected by the superficial blood contamination. In addition, diffuse reflectance intensity (Rd) between 650 nm and 800 nm from white/gray matter was significantly stronger than that from primary and secondary brain tumors and is used with the combined spectrum numerical value to enhance accurate determinations.