MONO-SPECIFIC POLYCLONAL ANTIBODIES AND METHODS FOR DETECTING CLOSTRIDIUM DIFFICILE TOXIN A

摘要

method is provided for the purification of Clostridium difficile Toxin antigen comprising reacting impure Toxin with immobilized mono-specific polyclonal antibodies. The polyclonal antibodies are coupled to a hydrazide group containing matrix such as hydrazide activated agarose gel. The immobilized antibody is specific for Toxin and will greatly purify Toxin from a Toxin containing solution. Antibodies raised to Toxin purified according to the method are of higher activity than antibodies produced from prior art purified Toxin.