ROLLING CIRCLE AMPLIFICATION DETECTION OF RNA AND DNA
摘要
Rolling circle amplification (RCA) has been useful for detecting point mutations in isolated nucleic acids, but its application in cytological preparations has been problematic. By pretreating cells with a combination of restriction enzymes and exonucleases, we demonstrate RCA in solution and in situ to detect gene copy number and single base mutations. It can also detect and quantify transcribed RNA in individual cells, making it a versatile tool for cell-based assays.
申请公布号
WO0250310(A3)
申请公布日期
2004.03.04
申请号
WO2001US49780
申请日期
2001.12.18
申请人
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
发明人
CHRISTIAN, ALLEN, T.;PATTEE, MELISSA, S.;ATTIX, CRISTINA, M.;TUCKER, JAMES, D.