| 摘要 |
<p>The present invention relates to a method for determining a nucleotide polymorphism, particularly a single nucleotide polymorphism (SNP) in a nucleic acid target. The method comprises the steps of: (a) providing a nucleic acid template to be analyzed, (b) annealing at least one primer to the nucleic acid template, wherein the 3'-end of the primer is located upstream of a nucleotide polymorphism to be analyzed, (c) sequence-specific elongating the primer by incorporating at least one labelled nucleotide into the primer, (d) degrading the nucleic acid template under conditions, wherein the elongated starting primer is substantially stable against degradation, and wherein the elongated primer is liberated from the nucleic acid template and (e) detecting labels incorporated into the primer which are characteristic for the nucleotide polymorphism to be analyzed.</p> |
