发明名称 |
Polypeptide nucleic sequences exported from mycobacteria, vectors comprising same and uses for diagnosing and preventing tuberculosis |
摘要 |
A recombinant screening, cloning and/or expression vector that replicates in mycobacteria, comprising: a replicon functional in mycobacteria; a selectable marker; and a reporter cassette comprising (a) a multiple cloning site (polylinker), (b) optionally a mycobacterial transcription terminator upstream of the polylinker, (c) a coding sequence from a gene coding for a protein expression, export and/or secretion marker, the coding sequence lacking its start codon and regulatory sequences, and (d) a coding sequence from a gene coding for a cis- acting promoter activity marker, the coding sequence having its start codon. Also covered is a method for screening mycobacterial nucleotide sequences to detect sequences coding for exported and/or secreted polypeptides that may be induced or repressed during infection, promoter and/or (other) regulatory sequences associated with such coding sequences, especially sequences that permit or enhance the export and/or secretion of such polypeptides, or all or part of genes coding for such polypeptides, using a vector as above.
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申请公布号 |
NZ519053(A) |
申请公布日期 |
2004.02.27 |
申请号 |
NZ19980519053 |
申请日期 |
1998.08.04 |
申请人 |
INSTITUT PASTEUR |
发明人 |
PELICIC, VLADIMIR;GUIGUENO, AGNES;GOGUET DE LASALMONIERE, YVES;GICQUEL, BRIGITTE;PORTNOI, DENIS;LIM,ENG-MONG |
分类号 |
G01N33/50;A61K39/00;A61K39/04;A61K48/00;A61P11/00;A61P31/04;A61P31/06;C07K14/35;C07K16/12;C07K19/00;C12N1/21;C12N15/09;C12N15/31;C12N15/74;C12P21/02;C12Q1/02;C12Q1/68;C12R1/32;G01N33/53;(IPC1-7):C12N15/74;A61K31/48 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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