发明名称 |
A kit that detects directly the pathogenicity of the pathogenic influenza A subtype H5 virus |
摘要 |
<p>Current methods for detecting influenza A subtype H5 virus, for example cell culture, haemagglutination- inhibition, fluorescent antibody and enzyme immunoassay, and reverse transcriptase polymerase chain reaction (RT- PCR) may have the disadvantages of low sensitivity and low specificity. Furthermore, such methods are relatively difficult to use, and may not be suitable for routine detection on a daily basis. A kit for detecting H5 virus is described. The kit provides a user-friendly alternative that is relatively more sensitive and specific to H5 virus. The kit utilizes two specifically designed primers A and B for the replication of H5 virus, and a specific capture probe for immobilizing the amplified viral RNA. An additional primer C is also designed for the detection of pathogenic H5 virus. The detection of H5 virus by the detection kit may be accomplished within one day.</p> |
申请公布号 |
NZ525190(A) |
申请公布日期 |
2004.02.27 |
申请号 |
NZ20010525190 |
申请日期 |
2001.09.27 |
申请人 |
HONG KONG DNA CHIPS LTD |
发明人 |
YU, ALBERT CHEUNG HOI;SO, KA-LUN;KO, LUNG-SANG;LAU,LOK-TING |
分类号 |
G01N33/53;C07H21/04;C12N15/09;C12Q;C12Q1/68;C12Q1/70;C12R;C12R1/92;C12R1/93;G01N33/569;(IPC1-7):C12Q1/70 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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