| 摘要 |
PCT No. PCT/FR94/01129 Sec. 371 Date Jun. 4, 1996 Sec. 102(e) Date Jun. 4, 1996 PCT Filed Sep. 27, 1994 PCT Pub. No. WO95/09231 PCT Pub. Date Apr. 6, 1995The invention relates to the field of biology and, in particular, to the field of cellular biology. The invention concerns a novel glucose-sensitive cell line designated beta cell line (INS-I) expressing glucokinase and the glucose carrier Glut 2 at levels comparable with those of normal beta cells but which is, in addition, incapable of IGF-II expression-dependent proliferation because of genetic manipulation. The invention also concerns a method for the production of said novel cell line, its aggregation in the form of a pseudoislet, its immobilization in a biocompatible hydrogel and its hardening by means of a hardening solution. Application in insulin-secreting beta -cell transplants.
|
