发明名称 Hydrophilic chemilumescent acridinium labeling reagents
摘要 In accordance with the present invention, it has been discovered that introduction of hydrophilic sulfoalkyl substituents and/or hydrophilic linkers derived from homocysteic acid, cysteic acid, glycine peptides, tetraethylene oxide, and the like, offset the hydrophobicity of the acridinium ring system to produce a more soluble label which can be attached to an antibody at higher loading before precipitation and aggregation problems are encountered. Additional compounds described herein contain linkers derived from short peptides and tetraethylene oxide which increase aqueous solubility due to hydrogen bonding with water molecules. The present invention also embraces reagents for multiple acridinium labeling for signal amplification composed of a peptide bearing several acridinium esters with sulfonate groups at regularly spaced intervals for increased solubility. The invention also embraces assays employing the above-described compounds.
申请公布号 US2004038294(A1) 申请公布日期 2004.02.26
申请号 US20020225084 申请日期 2002.08.20
申请人 QUEST DIAGNOSTIC INVESTMENTS INCORPORATED 发明人 EVANGELISTA RAMON;GARRITY MARTHA
分类号 C07D219/04;C07D401/12;C09B15/00;G01N33/533;(IPC1-7):G01N33/53;C07D219/08 主分类号 C07D219/04
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