| 摘要 |
Three-dimensional (3D) reconstruction of a cell includes adjusting a current set of projection images according to a priori knowledge to produce adjusted projection images, for example, based on probability masks and/or Bayesian analysis of multiple similar objects in the same sample. A reconstruction algorithm processes the adjusted projection images to generate a 3D image. The 3D image is further adjusted according to the a priori knowledge to generate an adjusted 3D image. Criteria for process completion are applied to determine whether the adjusted 3D image is adequate. Otherwise, a set of pseudo projections are computationally created at the same projection angles as the current set of projection images and then compared to the current set of projection images to produce a set of new projections, wherein the new projections are input again to the reconstruction algorithm and the steps of the method are repeated until the adequacy criteria are met. |
