| 摘要 |
The present invention provides a highly sensitive and practical method for the determination of and the separation of 5-hydroxycreatinine from a sample of bodily fluid. The method of the present invention is useful as a method for the testing of renal dysfunction, such as uremia, chronic nephritis and closure of the urinary tract, etc. A separation solvent of pH 4.1 to 4.6 is used in a high performance liquid chromatography step that is carried out using a strongly acidic cation-exchange resin, such as a styrene-divinylbenzene resin. The determination sensitivity is a concentration of only about 0.02 muM, whereby 5-hydroxycreatinine in blood of healthy persons can be determined for the first time. Moreover, only one separation solvent, such as a citrate, need be used and the cycle time for analysis is only about 14 minutes per measurement. Thus, a practitioner using one set of HPLC equipment is able to carry out about 100 determinations per day.
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