| 摘要 |
The present invention relates to an assay method for identifying an agent that modulates the electrical property of a membranous sample comprising the steps of: (a) providing an electrophysiological measurement apparatus; (b) contacting one or more membranous samples with a compartment of the electrophysiological measurement apparatus containing an aperture; (c) forming a seal between the membranous samples and the aperture in the presence of a low ion concentration seal phase internal solution; (d) exchanging the low ion concentration seal phase internal solution for an access phase internal solution; (e) establishing electrical access, via the aperture, to the internal compartment of the membranous sample; and (f) taking electrophysiological recordings before and after incubation of the cell with one or more agents; wherein a difference between: (i) the electrophysiological recording in the presence of the agent; and (ii) the electrophysiological recording in the absence of the agent is indicative of an agent that modulates the electrical property of a membranous sample. |
