Способ конструировани рекомбинантных штаммов микроорганизмов, экспрессирующих прокариотические и эукариотические гены, и их использование дл получени целевых продуктов

摘要

FIELD: biotechnology, genetic engineering, microbiology. ^ SUBSTANCE: invention relates to a method for construction of recombinant strains of microorganisms that provides expression of the target prokaryotic or eucaryotic gene in any strain of microorganism maintaining replication of vector DNA. Based on the multicopy plasmid pLF1311 a vector is constructed providing carrying out easy selection of cloned sequences by phenotype trait of microorganisms carrying it and to express their. Using this vector the probiotic starin Lactobacillus sp. BPA3 (pLF-SL2) is constructed that expresses eucaryotic gene encoding somatoliberin. This invention is used for the development of probiotics and ferments - preparations based on live cultures of microorganisms. ^ EFFECT: improved method for construction. ^ 1 dwg, 2 ex