DEVELOPING METHOD FOR SEPARATING D-AMYGDALIN AND NEOAMYGDALIN BY REVERSED-PHASE HPLC AND OPTIMUM CONDITIONS FOR INHIBITION OF RACEMIZATION OF AMYGDALIN

摘要

The present invention relates to the developing method for separating D-amygdalin and neoamygdalin by reversed-phase HPLC method and optimum condition for inhibition of racemization of amygdalin. In boiling aqueous solution, D-amygdalin usually begins to convert into neoamygdalin ineffective against cancer. Therefore, it has been necessary to examine optimum conditions for prevention of racemization. In the present invention, we developed reversed-phase HPLC method by C18 column to separate D-amygdalin and neoamygdalin to baseline resolution. Also, we established optimum conditions to inhibit conversion to neoamygdalin by changing pH. On the basis of these results, we applied to Persicae semen generally used in oriental preparations, and established optimum conditions to inhibit conversion to neoamygdalin of D-amygdalin in Persicae semen.