Selectable genetic marker for use in pasteurellaceae species

摘要

The present invention provides a nucleic acid encoding nicotinamide phosphioribosyltransferase (NadV) from a V-factor independent bacterium and provides methods for using the gene as a selection marker for constructing recombinant bacteria from V-factor dependent bacteria The method is an improvement over methods which rely on nucleic acids which confer antibiotic resistance for constructing recombinant bacteria. Methods for constructing attenuated recombinant (Actinobacillus pleuropneumoniae ) using the selection method of the present invention are also provided.