| 摘要 |
A eukaryotic bicistronic expression system for in vivo biotinylation of a protein is disclosed. The bicistronic expression system is based upon a polynucleotide which comprises a nucleic acid encoding a fusion protein made up of a selected protein and a biotinylation peptide, a nucleic acid sequence coding for an internal ribosome entry site and a nucleic acid sequence encoding a biotin ligase. Also disclosed are vectors and host cells containing the nucleic acid as well as methods for preparing a biotinylation protein and kits comprising the nucleic acid.
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