Dual condensation/epimerization domain in non-ribosomal peptide synthetase systems

摘要

The present invention relates to domains of non-ribosomal peptide synthetases (NRPSs) that exhibit dual condensation and epimerization activities. The "dual condensation/epimerization NRPS domains" of the present invention allow for the incorporation of non-proteinogenic substrates, such as D-amino acids, into peptide products. These dual condensation/epimerization NRPS domains may further be used to modify the stereochemistry of synthesized peptides at selected amino acid sites.