NOVEL CRYSTALLINE TRYPTASE AND UTILIZATION THEREOF

摘要

It is intended to provide a compound array, a library or a pharmacore of a compound which is obtained by subjecting a natural tryptase or a recombinant tryptase in the undenatured state to sugar chain cleavage and shows uniformity by Coomassie-staining in SDS-PAGE, and a screening method or a drug designing method of a compound having a tryptase inhibitory activity with the use of the same. Thus, tryptase crystals which sustain the activity and the sugar chain of which has been cleaved or crystals of a complex with the tryptase and a substance having an affinity thereto can be easily obtained. Using these crystals, a compound array, a library or a pharmacore of a compound having a tryptase inhibitory activity can be easily obtained. By using them, moreover, a compound having a tryptase inhibitory activity can be screened or drug-designed. Thus, a candidate compound can be appropriately obtained.