| 摘要 |
According to prior art, a system with immobilized DNA is used in the fields of medicine, environmentology or criminology as an analytical tool in the analysis of nucleic acid. According to the invention, the immobilized DNA is provided with a biocatalytically active marker, such as an enzyme, and with an inhibitor substance which reversibly inhibits catalytic activity, or in addition to the immobilized biocatalytic marker, the immobilized DNA is provided with a substance which can reversibly inhibit the catalytic activity of the marker. Alternatively, an immobilized biocatalytically active marker can be provided with DNA as a scavenger which includes a substance as an inhibitor which can reversibly inhibit the activity of the marker. In another alternative, it is possible to use a complex consisting of a molecule binding double-stranded DNA and a substance as an inhibitor which can reversibly inhibit the activity of the marker by interacting with the immobilized biocatalytically active marker. In all cases, the inhibitor or compound consisting of an inhibitor and a molecule which can bind double-stranded DNA interacts with the biocatalytically active marker and defines the inactive state of the system. When the DNA, which is to be analysed, is bonded,especially hybridized, to the DNA scavengers, the interaction between the biocatalytically active marker and the inhibitor is cancelled as a result of the formation of the double strand. The system is thus shifted from a first state into a second state defining the active state. A carrier (1) with integrated microelectrodes (5, 5') is provided in the associated device, whereby the enzyme is either immobilized therein or is contained in a polymer network in the vicinity of the microelectrodes (5, 5'). |
