| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To provide a method allowing production of recombinant retroviruses (retroviral vector particles) from producer cells which are safer and of higher titer than normal. <P>SOLUTION: A method for selecting a packaging cell producing a high level main factor selected from the group consisting of packaging proteins and target gene products comprises (a) supplying a genome comprising a main gene expressing the main factor in a cell and a selectable gene expressing a selectable protein in a cell to the packaging cell, herein the selectable gene is arranged in the downstream of the main gene and as a result is expressed at a lower level than that of the main gene, (b) exposing the packaging cell to a selection factor enabling identification of a cell expressing the selectable protein at a critical level, and (c) detecting a packaging cell expressing a higher level main factor. <P>COPYRIGHT: (C)2004,JPO</p> |
